SOP Title: Operation, Cleaning and Calibration of TLC UV Cabinet
- Objective:
To lay down the procedure for Operation, Cleaning and Calibration of TLC UV Cabinet.
- Scope:
This SOP is applicable for Operation, Cleaning and Calibration of TLC UV Cabinet located in Quality Control Laboratory.
- Responsibility:
- Quality Control: To prepare and review the SOP. To follow the procedures laid down for Operation, Cleaning and Calibration of TLC UV Cabinet as per this SOP.
- Engineering Department: To carry out preventive maintenance as per schedule and procedure defined.
- Quality Assurance Department: To review and approve the SOP and Annexures.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department
- Procedure:
| 5.1 | Safety Precautions/Instructions: | |
| 5.1.1 | Ensure all the electrical connections are properly earthed. Ensure the power plug of the TLC UV Cabinet is fixed in the socket properly. | |
| 5.1.2 | Wear personal protective equipments such as hand gloves during operation of equipment. | |
| 5.1.3 | Ensure that the ON/OFF switch is working properly by switching “ON” the TLC UV Cabinet. | |
| 5.2 | Operation: | |
| 5.2.1 | Check the validity of calibration before starting any operations. | |
| 5.2.2 | Ensure that the equipment should be cleaned before operation. | |
| 5.2.3 | Switch ‘ON ‘the main electrical switch. | |
| 5.2.4 | Open the door of ultra violet cabinet. | |
| 5.2.5 | Keep the thin layer chromatography plate inside the cabinet. | |
| 5.2.6 | UV cabinet is suitable for wavelength 254 nm and 365 nm. | |
| 5.2.7 | Switch ON the required UV lamp, as per standard testing procedure. | |
| 5.2.8 | View the TLC plate and check the results obtained. | |
| 5.2.9 | After completion, open the door of ultra violet cabinet and remove TLC plate. | |
| 5.2.10 | Switch OFF the UV lamp and switch OFF the main electrical switch. | |
| 5.2.11 | Update the “Instrument/Equipment Usage Log Book” as per SOP. | |
| 5.3 | Cleaning Procedure: | |||||||||||||
| 5.3.1 | Clean the instrument with lint free cloth inside and outside before and after completion of the test. | |||||||||||||
| 5.4 | Calibration Procedure: | |||||||||||||
| Frequency: Once in a Year or after carrying out any major maintenance work. | ||||||||||||||
| 5.4.1 | Generate the A. R. No. on the “Instrument/Equipment Calibration Register” as per SOP number and give requisition to QA department for calibration formats of instruments on the due date of calibration. | |||||||||||||
| 5.4.2 | For issuance of calibration report refer QA SOP. | |||||||||||||
| 5.4.3 | Perform the calibration of instrument as per Annexure and procedure mentioned below. | |||||||||||||
| 5.4.4 | Preparation of 95% ethanol: Add 10 ml purified water to 200 ml volumetric flask and make up the volume with ethanol and mix. | |||||||||||||
| 5.4.5 | Preparation of 0.04% w/v of Sodium Salicylate in 95% ethanol: Weigh accurately 40 mg of Sodium Salicylate in clean and dry 100 ml volumetric flask add about 50 ml of 95% ethanol, shake to dissolve and make up the volume to 100 ml with 95% ethanol and mix. | |||||||||||||
| 5.4.6 | Preparation of 0.2% w/v of Sodium Salicylate in 95% ethanol: Weigh accurately 100 mg of Sodium Salicylate in clean and dry 50 ml volumetric flask add about 30 ml of 95% ethanol, shake to dissolve and make up the volume to 50 ml with 95% ethanol and mix. | |||||||||||||
| 5.4.7 | Draw a line on the Silica gel 60 F254 and Silica gel 60 TLC plate in pencil at 1.5 cm from the bottom of the plate. | |||||||||||||
| 5.4.8 | Spot with glass syringe 5 micro litre of 0.04%w/v solution on Silica gel 60 F254 and 0.2%w/v solution on Silica gel 60 TLC plate of sodium salicylate on the line drawn of TLC plate. | |||||||||||||
| 5.4.9 | Put identification mark for the spots and allow the spots to dry in air. | |||||||||||||
| 5.4.10 | Examine the plate at 254 nm for 0.04%w/v spot and 365 nm for 0.2%w/v spot. | |||||||||||||
| 5.4.11 | Follow the same procedure from point no. 5.2.2 to 5.2.10. | |||||||||||||
| 5.4.12 | Enter the details in calibration report. Ensure that the results obtained, comply with the acceptance criteria.
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| 5.4.13 | Update the “Instrument/Equipment Usage Log Book”, “Annual Calibration Schedule” and “Monthly Calibration Schedule” as per “Calibration of Instruments and Equipments”. | |||||||||||||
| 5.4.14 | Affix the “Calibration Status Label” on the Instrument as per SOP “Calibration of Instruments and Equipments”. | |||||||||||||
| 5.4.15 | If calibration activity is outsourced, the values are to be transcribed in the Annexure and external party certificate to be attached. | |||||||||||||
| 5.4.16 | If any breakdown occurs, follow procedure as per SOP number “Calibration of Instruments and Equipments”. | |||||||||||||
- Definitions / Abbreviations :
- Abbreviations:
| Abbreviation | Expansion |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| QA | Quality Assurance |
| 0C | Degree Celsius |
| No. | Number |
| w/v | Weight by volume |
| nm | nanometer |
| TLC | Thin Layer Chromatography |
| UV | Ultra violet |
*Note – Ready to use SOP available in “DOWNLOAD” Section.