SOP Title: Procedure for Operation and Cleaning of Microscope
- Objective:
To lay down the procedure for Operation and Cleaning of Microscope.
- Scope:
This SOP is applicable for Operation and Cleaning of Microscope located in the Microbiology Section.
- Responsibility:
- Quality Control: To prepare and review the SOP. To follow the procedures laid down for Operation and Cleaning of Microscope as per this SOP.
- Engineering Department: To carry out preventive maintenance as per schedule and procedure defined.
- Quality Assurance Department: To review and approve the SOP and Annexures.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department.
- Procedure:
| Sr. No | Procedure | |
| 5.1 | Safety Precautions/Instructions: | |
| 5.1.1 | Wear personal protective equipments such as protective hand gloves during operation of instrument. | |
| 5.1.2 | Be cautious when handling glass slides and cover-slips. | |
| 5.1.3 | Cover the microscope with the polythene bag provided. | |
| 5.2 | Operation Procedure: | |
| 5.2.1 | Place the microscope on a rigid, firm, non-shaking, flat surface. | |
| 5.2.2 | Adjust the low power objective, by rotating the revolving nose piece in position. | |
| 5.2.3 | Adjust the focus of light with the help of mirror and condenser. | |
| 5.2.4 | Now position the required objective (low power, high power or oil immersion) by moving the resolving nose piece. | |
| 5.2.5 | Place the slides on the stage, lower the objectives, while observing through the eye piece, till the field appears clearly. | |
| 5.2.6 | If the oil immersion objective is in use, place one drop immersion oil on the slide before placing it on the stage and then lower the oil immersion Objective just to touch the oil drop. | |
| 5.2.7 | Make the field clearer with the help of fine adjustment and then observe the slide. | |
| 5.2.8 | Slide can be moved in left, right, upper and lower direction to observe the complete smear area with help of slide stage screw and coarse adjustment. | |
| 5.3 | Cleaning Procedure: | |
| 5.3.1 | Clean the microscope carefully with clean dry lint free napkin or a lens paper. | |
| 5.3.2 | Clean the eye piece and nose piece with 70% v/v IPA applied on the lens paper. | |
| 5.3.3 | After completion of observation, remove the slide, clean the objective lens and condenser with 70% v/v of IPA on the lens paper. | |
| 5.3.4 | Cover the microscope with the polythene bag provided. | |
- Definitions / Abbreviations:
- Definitions:
- Breakdown: Any activity leading to operation of instrument other than the set parameters or unusual sound or vibration observed in the instrument.
- Preventive Maintenance: Maintenance activity performed to ensure that the instrument will function smoothly and to avoid breakdowns. These activities are performed as per predefined frequency.
- Abbreviations:
| Abbreviation | Expansion | |||
| No. | Number | |||
| v/v | Volume by volume | |||
| IPA | Iso Propyl Alcohol | |||
| QC | Quality Control | |||
| % | Percentage | |||
*Note – Ready to use SOP available in “DOWNLOAD” Section.