SOP Title-Operation and Cleaning of Microscope
- OBJECTIVE
To lay down the procedure for operation and cleaning of microscope.
- SCOPE
This SOP is applicable for operation and cleaning of microscope in Microbiology Lab.
- RESPONSIBILITY
3.1 QC Executive and above shall be responsible for implementation of this procedure.
3.2 QC Head / designee shall be responsible for compliance of this procedure.
- PROCEDURE :-
- Precautions :
- Check the cleaning status of microscope.
- Operation of Microscope:
- Switch ON the Mains Switch.
- Switch ON the microscope (Ensure that Intensity of light controller of the microscope is kept to minimum level every time before switching OFF or switching ON the microscope)
- Select the objective.
- Adjust the diaphragm & intensity controller to get sufficient light towards the stage micrometer.
- Place the slide to be examined, on the stage, underneath the lens.
- Set the magnification as per requirement.
- Focus the slide with the help of Coarse Adjustment Knob. (Coarse & Fine adjustments are coaxially mounted. The outer knob being for the coarse focusing and the smaller inner knob for fine focusing).
- Focus for clarity with the help of the fine adjustment knob and make observations.
- Adjust the ABBE condenser in upward or lower direction as per the chosen objective.
- For low magnification the condenser is moved to a lower position by provided knob and for higher magnification to upper position.
- Cleaning:
- After use, instrument shall be cleaned with lint free cloth/tissue paper by using 70% IPA.
- ABBREVIATION(s)
| Abbreviation | Full Description |
| SOP | Standard Operating Procedure |
| IPA | Isopropyl Alcohol |
*Note – Ready to use SOP available in “DOWNLOAD” Section.