SOP Title-Operation and Cleaning of Micropipette
- OBJECTIVE
To lay down the procedure for operation and cleaning of micropipette.
- SCOPE
This SOP is applicable for operation and cleaning of micropipette in Microbiology Lab.
- RESPONSIBILITY
3.1 QC Executive and above shall be responsible for Implementation of this procedure.
3.2 QC Head / designee shall be responsible for compliance of this procedure.
- PROCEDURE :
- Precautions;
- Check the cleaning status of micropipette.
- Check the calibration status of micropipette.
- Operation of micropipette:
- Adjust the required volume of micropipette by rotating the knobs provided..
- Attach tips to Micropipette by pressing at the base.
- Press the top central knob up to first step lock.
- Dip the Micro-pipette with tips in the solution in such way that only half of the tip will get dip.
- Then release the top central knob, it will suck the solution.
- Then remove the Micro-pipette from the dipped solution.
- Dispense sucked solution in required tube/container by pressing full top central knob up to second step lock & release the knob.
- To remove the tip attached, press top side knob.
- Use separate and sterilized tips for each product/materials.
- Cleaning:
- Clean the micropipette with the help of tissue paper/dry lint free cloth.
- After handling of culture, micropipette shall be cleaned with 70% IPA.
- ABBREVIATION(s)
| Abbreviation | Full Description |
| SOP | Standard Operating Procedure |
| IPA | Isopropyl Alcohol |
*Note – Ready to use SOP available in “DOWNLOAD” Section.