Calibration of Total Organic Carbon Analyzer (TOC)

Calibration of Total Organic Carbon Analyzer (TOC)

 

 

• PROCEDURE
  • Operate the instrument as per SOP.
  • Ensure that instrument is clean before start the calibration, if not then clean as per cleaning procedure.

• Calibration Procedure:

  • KHP (Potassium hydrogen phthalate) solution :-

Weigh accurately about 212.5 mg of KHP, transfer it to a 100 ml volumetric flask and make up the volume 100 ml with purified water. Take 1 ml of this solution in a 100 ml volumetric flask and make up the volume 100 ml with purified water. This will give 10000 ppb KHP solution. (Stock Solution)

• Standard preparation of 800ppb KHP (Potassium hydrogen phthalate):

Pipette 20 ml stock solution in 250ml volumetric flask and make up the volume up to 50ml.

• Standard preparation of 500ppb KHP (Potassium hydrogen phthalate):

Pipette 5ml stock solution in 100ml volumetric flask and make up the volume up to 100ml.

• Standard preparation of 250ppb KHP (Potassium hydrogen phthalate):

Pipette 5ml stock solution in 200ml volumetric flask and make up the volume up to 200ml.

 

• System Suitability Procedure:

• Sucrose solution :-

  • Dry the primary standard Sucrose at 105⁰C for 60 – 90 minutes in a hot air oven.

Weigh accurately about 23.8 mg of this sucrose when it comes to room temperature and dissolve it in 200 ml volumetric flask. Make up the volume 200 ml with purified water (50 ppm sucrose solution).Use this solution as a stock solution.

• Take 1 ml of this solution in a 100 ml volumetric flask and make up the volume 100 ml. (500 ppb solution of sucrose)

• 1,4-Benzoquinone solution :-

  • Weigh accurately about 37.5 mg of 1, 4 – Benzoquinone and add it to a 500 ml volumetric flask. Make up the volume 500 ml with purified water. (50 ppm 1,4 – Benzoquinone solution)
  • Take 1 ml of this solution in a 100 ml volumetric flask and make up the volume 100 ml. (500) ppb solution of 1, 4 – Benzoquinone.
  • Use purified water as zero ppb solution.
• Acceptance criteria
  • SD Should be less than 5.0
  • CV should be less than 3% (Either SD or CV limits will be accepted).
  • Calibration: Correlation coefficient (r²) shall be greater than 0.98.
  • System suitability: The % recovery should be between 85% to 115%.
  • After the successful completion of calibration. Calibration label shall be affixed on the instrument as per Annexure.
  • If the Calibration parameters are out of limit then inform Head QC.
  • Frequency: Perform system suitability once in 15 days ± 2 days and calibrate the instrument quarterly.

 

• ABBREVIATION(s)

Abbreviation	Full Description
QC	Quality Control
SOP	Standard Operating Procedure
No.	Number
SD	Standard deviation
CV	Coefficient of variance
ppm	Parts per million
ppb	Parts per billion

 

 

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