Calibration of UV Cabinet
- PROCEDURE :-
- Frequency: Calibrate the instrument quarterly.
- Operate the instrument as per operation of UV cabinet SOP.
- Apply 5µl, 04% w/v solution of sodium salicylate AR and 0.2% w/v solution of sodium Salicylate AR in ethanol (95%), on 10 x 5 cm plate coated with silica gel G and view the spot under wavelength of 254 nm and 365 nm respectively.
- Acceptance criteria:
Intensity of the spot should be clear and visible of 0.04% w/v solution of sodium salicylate AR and 0.2% w/v solution of sodium Salicylate AR in ethanol (95%) at 254 nm and 365 nm respectively.
- Calibration Report of UV Cabinet shall be prepared as per Annexure.
- After the successful completion of calibration. Calibration label shall be affix on the instrument as per SOP.
- If the Calibration parameters are out of limit affix the Out of Calibration label and Inform to QC Head/Designee
- Activity shall be recorded in the Instrument usage log book.
- ABBREVIATION(s)
| Abbreviation | Full Description |
| %w/v | Percent Wight/Volume |
| cm | Centimeter |
| UV | Ultra-Violet |
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