SOP Title: Area Cleaning and Sanitation of Microbiology–Section
- Objective:
To lay down a procedure for Area Cleaning and Sanitation of Microbiology Section
- Scope:
The scope of this SOP is applicable for Area Cleaning and Sanitation of Microbiology Section of Quality Control Department.
- Responsibility:
- Quality Control: To prepare and review the SOP. To follow the procedures laid down for the Procedure for Area Cleaning and Sanitation Section as per this SOP.
- Quality Assurance Department: To review and approve the SOP and Annexure.
- House Keeper: To follow the procedures laid down for the Procedure for Area Cleaning and Sanitation Section as per this SOP.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department.
- Procedure:
| 5.1 | Preparation of disinfectant solution: | |
| 5.1.1 | Dettol – 2.5% v/v solution in purified water. i.e. 2.5 part dettol diluted to 97.5 parts of purified water | |
| 5.1.2 | Savlon – 2.5% v/v solution in purified water. i.e. 2.5 part savlon diluted to 97.5 parts of purified water. | |
| 5.1.3 | IPA – 70% v/v solution in purified water. i.e. 70 parts IPA diluted to 30 parts of purified water. | |
| 5.2 | Procedure for Cleaning and Sanitisation of Floor: | |
| 5.2.1 | The mopping operation is to be carried out before start of activity and after completion of analysis or at the end of the shift. | |
| 5.2.2 | Use a clean dry lint free mop/cloth and remove any dust. | |
| 5.2.3 | Use a lint free mop and dedicated buckets/containers for different disinfectants. | |
| 5.2.4 | Clean the area with purified water, allow to dry and then mop with disinfectant solution. | |
| 5.2.5 | Use freshly prepared disinfectant solution for mopping the floors. | |
| 5.2.6 | Measure the required amount of disinfectant and add purified water to make up final desired volume. | |
| 5.2.7 | Moping should be carried out as per the sequence: | |
| 5.2.8 | LAF room to be cleaned first with purified water, then the third and second air lock followed by the incubation room and first airlock. Finally mop the media preparation room. | |
| 5.2.9 | Follow the same pattern for disinfectant solution. | |
| 5.2.10 | Rotate the disinfectants every week to avoid the development of resistance by microorganisms. | |
| 5.2.11 | For the first and third week use dettol and second and fourth week use savlon. | |
| 5.3 | Procedure for Area Cleaning: | |
| 5.3.1 | Perform the area cleaning and sanitation when there is no activity. | |
| 5.3.2 | De-dust the surfaces of the ceilings, walls, pass boxes and exposed parts of the equipments with the aid of dry clean cloth twice a week before mopping. Preferably on Monday and Thursday (if Monday/Thursday is a holiday than next working day). | |
| 5.3.3 | Record the activity in Annexure ‘Area cleaning and sanitation record’. | |
| 5.3.4 | For cleaning glasses, use Colin as cleaning solution every week. | |
| 5.3.5 | Tables, pass boxes and other surfaces shall be cleaned with a lint free cloth using 70%IPA solution. | |
| 5.4 | Cleaning Frequency: | |
| 5.4.1 | Floor – twice a day. Walls, Glass, Ceilings – twice in a week | |
- Definitions / Abbreviations:
- Definitions:
- Abbreviations:
| Abbreviation | Expansion |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| QA | Quality Assurance |
| % | Percent |
| v/v | volume by volume |
| IPA | Isopropyl Alcohol |
| No. | Number |
*Note – Ready to use SOP available in “DOWNLOAD” Section.