Calibration of Colony Counter

Calibration of Colony Counter

• PROCEDURE
• PRECAUTIONS
• Check the cleaning status of colony counter.
• Operation of Colony Counter:
• Connect the cord of the instrument to the mains socket and switch ON the main supply.
• Switch ON the “POWER” button. The internal light shall be flashed ‘ON’ & the digital screen shall display ‘0000’ figure.
• Reset the counter if required by pressing reset button.
• Place a blank petridish without agar media on the graduated glass plate and fix the magnifying lens by focusing on the plate at the desired level.
• Remove the cap of marker.
• Press the marker gently on the petridish at random locations.
• Instrument shall beep on every pressing.
• Press the marker on the petridish counting manually for a count of 25, and check the count registered on the display. The registered count on the display should be 25.
• Repeat the same procedure for 50, 100, 150, 200 and 250 counts on another petridishes. The display should register a count of 50, 100, 150, 200 and 250 respectively.
• Record shall be maintained as per annexure.
• Frequency: Once in a month.

• ABBREVIATION(s)

“End of Document”