SOP Title: Operation, Cleaning and Calibration of Fume Hood
- Objective:
To lay down the procedure for Operation, Cleaning and Calibration of Fume hood.
- Scope:
This SOP is applicable for Operation, Cleaning and Calibration of Fume hood located in Quality Control Laboratory.
- Responsibility:
- Quality Control: To prepare and review the SOP. To follow the procedures laid down for operation, cleaning and calibration of Fume hood as per this SOP.
- Quality Assurance Department: To review and approve the SOP and Annexure.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department
- Procedure:
| 5.1 | Safety Precautions/Instructions: | |
| 5.1.1 | Ensure all the electrical connections are properly earthed. Ensure the power plug of the Fume hood is fixed in the socket properly. | |
| 5.1.2 | Wear personal protective equipment’s such as protective hand gloves, nose masks and over gown during operation of the fume hood. | |
| 5.1.3 | Do not disturb the air flow or switch off the fume hood if analysis are not completed. | |
| 5.1.4 | Avoid placing head inside fume hood. Close the sash/door as to safe opening height/Minimum Height i.e. 18 cm from the working platform level when working in the fume hood. | |
| 5.1.5 | Avoid the rapid movement of Sash/door of fume hood. This can cause air currents drawing contaminants out of the fume hood. | |
| 5.1.6 | Avoid raising the sash/door too quickly. Raise the sash/door slowly and allow the air velocity to increase. | |
| 5.1.7 | Do not work by opening sash/door higher than the maximum established working height. Close the Sash/Door completely when not working at the fume hood. | |
| 5.1.8
|
Avoid storage of larger amount of chemicals in the fume hood. The storage of chemicals in fume hood may block airflow. Use underneath cabinet designed for chemical storage. | |
| Note: While working with the chemicals, position the sash/door at safe opening height/minimum height. | ||
| 5.1.9 | Do not place any spark source (i.e. electrical receptacles) inside the hood when flammable liquid or gasses are present e.g. Ether, IPA, Acetonitrile, Ethanol, Methanol, LPG, Hydrogen gas etc. | |
| 5.2 | Operation Procedure: | |
| 5.2.1 | Switch ‘ON’ the main switch of MCB box to supply electricity to the fume hood and blower. | |
| 5.2.2 | To Start the lights, put ‘ON’ the main switch provided on the side wall and then switch ‘ON’ the button which is provided on the right side of the fume hood.
Note: Two power switches are provided for the connection of Instruments. |
|
| 5.2.3 | Start the Air Blower/Air Exhaust by pressing green button on the Starter switch. | |
| 5.2.4 | Verify the Air Blower/Exhaust system is operating properly before use by checking of current of air flow or by generating smoke in fume hood. | |
| Note: Back wall of the fume hood is provided with the holes for exhaust. | ||
| 5.2.5 | Keep instruments minimum 15 cm away from the sash/door while using fume hood. | |
| 5.2.6 | Never work with sash opening higher than the established working height. Sash is provided with mark of minimum and maximum of usage height. | |
| Note: Do not close sash/Door fully. If sash is fully closed then blower will not get the required air and vacuum will be created in the fume hood. | ||
| 5.2.7 | Left side of the fume hood is provided with the 3 knobs i.e. for water, for air and for gas. Valves can be open/close by rotating Knob left/right side as per the need. | |
| 5.2.8 | Opening of these valves is provided inside the fume hood. | |
| 5.2.9 | Fume hood is provided with water tap along with the Basin with drainage connection. | |
| 5.2.10 | Avoid excessive heat within the fume hood. | |
| 5.2.11 | Cupboard is provided downside of the fume hood to store the Liquid chemicals and concentrated acids etc. | |
| 5.2.12 | After the analysis, allow to run the blower at least 5 minutes to remove toxic gas or fumes from fume hood and switch “OFF” the air flow blower. | |
| 5.2.13 | After cleaning switch “OFF” the light and main switch. | |
| 5.2.14 | Cleaning should be done as per below mention cleaning procedure. | |
| 5.3 | Cleaning Procedure: | |
| 5.3.1 | Cleaning should be done before starting and after the completion of analysis. | |
| 5.3.2 | Clean the chemical spills on working surface as soon as possible by washing with plenty of water. Clean the dust on sash and side wall with dry cloth. | |
| 5.3.3 | Minimize the excessive fumes by controlling the process of analysis. For Example- In Sulphated Ash testing, drop wise addition of Sulphuric Acid will avoid excessive fumes. | |
| 5.4 | Validation: | |
| Frequency: Once in a year or after carrying out any major maintenance work | ||
| 5.4.1 | Fume Hood shall be validated for Face Velocity/Air Velocity Test. The face velocity should be checked at different five positions as shown in the Annexure “ Location Chart” | |
| 5.4.2 | Acceptance Criteria :- Average of five reading should be within 0.4-0.6 m/sec ( i.e. ~ 80- 120 fpm)
*Note: – Acceptance Criteria reference from ANSI Standard. Record the reading in Annexure. |
|
| 5.4.3 | Validation shall be carried out internally or by external agency. Refer SOP “Calibration of Instruments and Equipment for documentation. | |
| 5.5 | Preventive Maintenance | |
| Frequency: Every Quarterly | ||
| 5.5.1 | Perform the preventive maintenance as per Annexure | |
- Definitions/Abbreviations:
- Definitions:
- Breakdown: Any activity leading to operation of fume hood other than the set parameters and/ or unusual sound or vibration observed in the fume hood.
- Calibration: Tests performed to ensure that the equipment is functioning as per set parameters. These tests are performed as per predefined frequency and also after any major repair or replacement of parts.
- Abbreviations:
| Abbreviation | Expansion |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| QA | Quality Assurance |
| No. | Number |
| PM | Preventive Maintenance |
| cm | Centi meter |
| m/sec | Meter Per Second |
| fpm | Feet Per Minute |
Fume Hood Calibration/Validation Report
Observed Anemometer Reading at Five different position
| Position
No. |
Anemometer Reading
at given position(m/sec) |
Acceptance Criteria |
| I | Average of five reading should be within 0.4-0.6 m/sec | |
| II | ||
| III | ||
| IV | ||
| V |
Average Reading = Sum of all Anemometer reading
5
= _____________________
5
= m/sec
Remark: The result Complies/Does not complies as per acceptance criteria.
Performed By: ________________ Date: _______________
Checked By: ______________ Date: ______________
The face velocity should be checked at five different positions as shown below.
BACK SIDE
|
( I ) ( II )
(III )
( IV ) ( V ) |
FRONT SIDE
*Note – Ready to use SOP available in “DOWNLOAD” Section.