SOP Title: Operation, Cleaning and Calibration of Centrifuge Apparatus
- Objective:
To lay down the procedure for Operation, Cleaning and Calibration of Centrifuge Apparatus.
- Scope:
This SOP is applicable for Operation, Cleaning and Calibration of Centrifuge Apparatus located in Quality Control Laboratory.
- Responsibility:
- Quality Control: To prepare and review the SOP. To follow the procedures laid down for Operation, Cleaning and Calibration of Centrifuge Apparatus as per this SOP.
- Engineering Department: To carry out preventive maintenance as per schedule and procedure defined.
- Quality Assurance Department: To review and approve the SOP and Annexures.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department.
- Procedure:
| 5.1 | Safety Precautions/Instructions: | |||||||||||||||||||||||
| 5.1.1 | Ensure all the electrical connections are properly earthed. Ensure the power plug of the centrifuge apparatus is fixed in the socket properly. | |||||||||||||||||||||||
| 5.1.2 | Ensure that the ON/OFF switch is working properly by Switching “ON”
the centrifuge apparatus. |
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| 5.2 | Operation: | |||||||||||||||||||||||
| 5.2.1 | Switch ON the main switch. Red light indicator on the equipment will glow. | |||||||||||||||||||||||
| 5.2.2 | Open the lid of centrifuge apparatus. | |||||||||||||||||||||||
| 5.2.3 | Four-centrifuge tube stands are provided inside the equipment. | |||||||||||||||||||||||
| 5.2.4 | Check that the centrifuge tubes are clean and dry before pouring sample solution in it. | |||||||||||||||||||||||
| 5.2.5 | Put the centrifuge tube containing sample solution in the centrifuge apparatus. | |||||||||||||||||||||||
| 5.2.6 | Ensure that the sample solution should not be more than 80% capacity of the centrifuge tube. | |||||||||||||||||||||||
| 5.2.7 | Place the centrifuge tubes in such positions that the opposite sides are balanced. | |||||||||||||||||||||||
| 5.2.8 | When using two centrifuge tubes they should be placed opposite to each other. | |||||||||||||||||||||||
| 5.2.9 | Turn the main knob to the ‘1’ position in clockwise direction. | |||||||||||||||||||||||
| 5.2.10 | Instrument will start at low speed (1000 rpm). Then increase the speed to position 2 (2000 rpm), 3 (4000 rpm), 4 (8000 rpm), 5 (10000 rpm) or as required. | |||||||||||||||||||||||
| 5.2.11 | After completion, slowly decrease the speed on the knob and then finally bring to OFF position. | |||||||||||||||||||||||
| 5.2.12 | Open the lid of centrifuge machine, remove the centrifuge tube and check for satisfactory results. | |||||||||||||||||||||||
| 5.2.13 | Switch OFF the main switch after completion. | |||||||||||||||||||||||
| 5.3 | Cleaning Procedure: | |||||||||||||||||||||||
| 5.3.1 | Clean the centrifuge machine externally and internally with clean lint free cloth. | |||||||||||||||||||||||
| 5.4 | Calibration Procedure: | |||||||||||||||||||||||
| Frequency: Once in a year by external agency or after carrying out any major maintenance work. | ||||||||||||||||||||||||
| 5.4.1 | Calibration is carried out by external agency as per SOP “Calibration of Instruments and Equipments”. | |||||||||||||||||||||||
| 5.4.2 | Refer Annexure for calibration test to be performed and acceptance criteria.
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| 5.4.3 | Generate the A. R. No. on the “Instrument/Equipment Calibration Register” as per SOP and give requisition to QA department for calibration formats of instrument/equipment on the due date of calibration. | |||||||||||||||||||||||
| 5.4.4 | For issuance of calibration report refer SOP “Control of documents, Preparation, Approval, Issuance and Maintenance. | |||||||||||||||||||||||
- Definitions / Abbreviations:
- Definitions:
- Breakdown: Any activity leading to operation of instrument/equipment other than the set parameters or unusual sound or vibration observed in the instrument/equipment.
- Calibration: Tests performed to ensure that the instrument/equipment is functioning as per set parameters. These tests are performed as per predefined frequency and also after any major repair or replacement of parts.
- Abbreviations:
| Abbreviation | Expansion |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| QA | Quality Assurance |
| No. | Number |
| A. R. No. | Analytical Reference Number |
*Note – Ready to use SOP available in “DOWNLOAD” Section.