SOP Title: Entry Exit Procedure in Microbiology Section
- Objective:
To lay down the written procedure for Entry Exit in Microbiology Section of Quality Control
Department.
- Scope:
This procedure is applicable for Entry Exit of working personals and visitors in the Microbiology
Section of Quality Control Department.
- Responsibility:
- Quality Control Department: To prepare and review the SOP. To follow the procedures laid down for Entry Exit Procedure in Microbiology Section of Quality Control Department as per this SOP.
- Quality Assurance Department: To review and approve the SOP and Annexures.
- Accountability:
Head Quality Control Department, Head Quality Assurance Department.
- Procedure:
| 5.1 | Precautions: | |
| 5.1.1 | The Good Laboratory Practices must be read prior to entering the microbiology section. By being knowledgeable about the procedures, analyst will assure their own safety. | |
| 5.1.2 | Person must sanitize their hands before entering the microbiology section and after working with potentially hazardous materials, before leaving the laboratory. | |
| 5.1.3 | Never wear open toed shoes because of the constant danger of cuts and infections from broken glass found on the lab floors and the possibility of chemical or microbial culture spills. | |
| 5.1.4 | Decontaminate work surfaces after completion of work and after any spill or splash of potentially infectious material with appropriate disinfectant. | |
| 5.1.5 | Do not take microbial cultures out of microbiological area since it may cause fatal infections if not handled properly. | |
| 5.1.6 | Ensure that single door is opened at a time. Do not open the next one until the previous one has been closed, to prevent the cross contamination. | |
| 5.2 | Entry Procedure for the Microbiology Section: | |
| 5.2.1 | Entry should be restricted in Microbiology Laboratory for other than concern personnel. | |
| 5.2.2 | Switch ‘ON’ the department lights. | |
| 5.2.3 | Check the differential pressure on magnehelic gauge is minimum 1mm before entering in Microbiology Laboratory. | |
| 5.2.4 | Before starting the work, ensure that the cleaning activities are completed in the presence of the house keeping personnel/head.
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| 5.2.5 | A person entering in the microbiology section of the Quality Control Department should enter in first cubical by pushing the door and remove the company footwear and disinfect hands with 70% IPA and should enter in second cubical by pushing the door and wear the sterilised over gown available in the box provided at second cubicle along with the sterile hand gloves, mask and headgear. Ensure that minimum part of the body gets exposed within the microbiology section. One over gown can be used for the day by the same person. | |
| 5.2.6 | Enter the third cubical. Disinfect the hands with disinfectant available in third cubical before entering the inoculation room. Push the door with the reverse side of the palm to prevent contamination. | |
| 5.2.7 | Verify the HVAC working condition by monitoring the temperature and Humidity of the section. If not, intimate the engineering department. | |
| 5.2.8 | Incubation room entry is provided through first cubical of change room. Push the door of incubation room and enter inside. | |
| 5.2.9 | There is no need of over gowning while entering in the media preparation room and incubation room. | |
| 5.2.10 | Protective nose mask, safety goggle, hand gloves and heat resistant gloves are required for autoclave handling and weighing of dehydrated media. | |
| 5.3 | Exit Procedure for the Microbiology Section: | |
| 5.3.1 | Disinfect the hands before leaving the inoculation room with 70 % IPA available in inoculation room, enter in third cubicle and disinfect hands with disinfectant available in the third cubicle, then enter into the second cubicle, remove the over gown and other accessories and keep it in the rack provided.
Used hand gloves should be discarded in Dustbin after every use. Then enter First cubicle, disinfect hands with 70 % IPA and then exit. |
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| 5.3.2 | After the Analysis is completed for the day, remove the used over gown and keep it in a container labelled as “Linen for washing” available in first cubicle to send it for washing at the end of shift. | |
| 5.3.3 | At the end of the analysis ensure that all the instruments/equipments used are switched ‘OFF’, except the ones which are to be kept ‘ON’ for 24 hours such
as incubators. Also clean the work benches before leaving the department. |
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| 5.3.4 | Used glassware’s shall be disinfected and cleaned as per SOP ‘Procedure for Cleaning of Used Glassware in Microbiology Section’. Broken glassware to be discarded into the box marked “Broken Glassware” and not into waste bins. | |
| 5.3.5 | Ensure the valve of gas cylinder used in inoculation room and the regulator valve of gas cylinder in microbiological section are closed, as mentioned in SOP ‘Procedure for operation of laminar flow work table’. | |
| 5.3.6 | Verify that all the chemicals, media, reagents, liquid solvents are properly closed and kept in their respective places after use and the water supply taps are also closed. | |
| 5.3.7 | During exit all doors should be opened and closed one by one, same as that of entry procedure. | |
| 5.3.8 | Ensure that lights are switched ‘OFF’ before leaving the section. | |
| 5.3.9 | All visitors should be accompanied by authorized company employee in Microbiology Section of Quality Control Department. | |
| 5.3.10 | All visitors should follow the same entry and exit procedure as mentioned above. | |
- Definitions/Abbreviations:
- Definitions :
- Abbreviations :
| Abbreviation | Expansion |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| QA | Quality Assurance |
| HVAC | Heating, Ventilating and Air Conditioning |
| LAF | Laminar Air Flow |
| LPG | Liquid Petroleum Gas |
*Note – Ready to use SOP available in “DOWNLOAD” Section.