SOP Title-Operation and Cleaning of Antibiotic Zone Reader

SOP Title-Operation and Cleaning of Antibiotic Zone Reader

• OBJECTIVE

To lay down the procedure for operation and cleaning of antibiotic zone reader.

• SCOPE

This SOP is applicable for operation and cleaning of antibiotic zone reader in Microbiology Lab.

• RESPONSIBILITY
• QC Executive and above shall be responsible for Implementation of this procedure.
• QC Head / designee shall be responsible for compliance of this procedure.
• PROCEDURE :
• Precautions
• Check the cleaning status of antibiotic zone reader.
• Check the calibration status of antibiotic zone reader.
• Operation of Antibiotic Zone Reader :
• Switch ‘ON’ the mains switch.
• Turn the left side drum to its maximum clockwise position, so that zero marking on the scale of the right side drum coin­cides with zero marking on the vernier scale.
• Keep the petri dish on the aluminium plate and rotate it manu­ally; so that one end of the zone image touches the mark line on the prism.
• Now turn the left side drum slowly, in anti-clockwise posi­tion, till the other end of the zone image touches the mark line on the prism.
• Note the reading directly on the right side scale.
• The scale is marked from 0 to 35 mm.
• The side scale having each division equal to 0.2 mm.
• Remove the petri dish and switch ‘Off’ the mains.
• record shall be maintained as per instrument usage log book.
• Cleaning:
• After use, Instrument shall be cleaned with 70% IPA by using tissue paper/clean lint free cloth.

• ABBREVIATION(s)

*Note – Ready to use SOP available in “DOWNLOAD” Section.