SOP Title-Operation of Gas Chromatography (GC)
- OBJECTIVE
To lay down the procedure for Operation of Gas Chromatography.
- SCOPE
This SOP is applicable to Operation of Gas Chromatography in Quality Control laboratory.
- RESPONSIBILITY
- QC personnel shall be responsible for implementation of this procedure.
- QC Head /designee shall be responsible for compliance of this procedure.
- OPERATION PROCEDURE :
- Operation of Gas Chromatography :
5.1.1 Check whether sufficient pressure is in zero air, hydrogen and nitrogen gas in the respective cylinders and on gas purification unit.
5.1.2 Check the tubes carrying gases are connected to the main instrument at their proper inlets.
5.1.3 Connect the required column between the injection port and detector.
5.1.4 Open the valves of Nitrogen, Zero air and Hydrogen at the gas purification unit.
5.1.5 Set the gas flow rate of Hydrogen, Zero air 5 kg/cm2.and Nitrogen 6 kg/cm2
5.1.6 Switch on the power supply to the instrument and press power ON from right side of instrument.
5.1.7 Also switch on the Head space from back side of it.
5.1.8 Mean while switch on the computer, which is connected with instrument.
5.1.9 Double click the GC solution software icon from the desktop. Then select operation.
5.1.10 Click the instrument 1 icon. The login menu will appears, input user ID than password than click ok.
5.1.11 Check the system on icon, than select the desired method form following manner.
Click file menu→Open method file→Select local disk D→Open GC method file→Select desired method file→than click open →than click the download parameters from real time window.
5.1.12 Click the instrument monitor icon from toolbar.
- Operation of Head space :
- 5.2 Creation of new method :
- 5.2.1.1 To enter in method mode, press MET key on the left side of the front screen.
5.2.1.2 The key MET will light and than enter method number 1,2 or 3 etc. & method kind C by pressing up, down, left and right keys.
5.2.1.3 Then press down key and enter analysis time (It is 5 minute greater than GC method.) by pressing left or right key.
5.2.1.4 Than press down key and enter Oven conditioning Temperature by pressing left or right keys.
5.2.1.5 Then press down key to change the incubation time inside the oven by pressing left and right keys (It is 5 minute less than analysis time).
5.2.1.6 Then press down key to reach the syringe conditioning temperature by pressing left and right keys.
5.2.1.7 Then press down key regular to reach at the end of method mode than press the enter key to save the method.
5.2.1.8 At last press MET key again to exit from method mode.
5.2.2 Creation of new sequence :
5.2.2.1 To enter in sequence mode, press SEQ key on the left side of the front screen.
5.2.2.2 Press down key and enter method number, which is created previously in method mode by pressing left and right keys.
- Press down key to enter the vial position on the sample tray that is defined as first sample and last sample, for example first sample-1.0 and last sample-1.3.
- At last press right key regular to select the END. When END will blink then press enter key.
- Press SEQ key again to exit the sequence mode.
- 5.2.3 Run the Head Space :
5.2.3.1 Put sample in vials sealed tightly with septa on the sample tray at suitable position, which is defined in headspace method.
5.2.3.2 When GC status will ready on the instrument monitor than click the batch processing menu at real time window and enter the vial NO., Sample name, Sample ID, then method file and data file.
- Save the batch processing at desired folder in local disk D.
- Go on head space main menu and select automatic run by pressing down key and enter in automatic run mode than press down key when drag will complete press START key and click start button from monitor simultaneously.
- Switch Off the instrument :
5.3.1 Before switching off the instrument, open the cooling method file from GC method and then click on “down load parameter”. This will cool off instrument.
- When GC status will ready than click “GC system off” then switch off all power switches.
- Close all the knob and regulator from gas purification unit. Also close the valve of all cylinders.
7.0 ABBREVIATION(s)
| Abbreviation | Full Description |
| QC | Quality Control |
| QA | Quality Assurance |
| SOP | Standard Operating Procedure. |
| GC | Gas chromatography |
| No. | Number |
| RT | Retention time |
*Note – Ready to use SOP available in “DOWNLOAD” Section.