Calibration of FTIR Spectroscope
1. Control of Resolution Performance:
| Parameters | Observations | Acceptance Criteria |
| The difference between the percentage transmittance at the transmission maximum at 2870 cm-1 and that at the transmission maximum at 2849.5 cm-1. | Should be greater than 18% | |
| The difference between the percentage transmittance at the transmission maximum at 1589 cm-1 and that at the transmission maximum at 1583 cm-1. | Should be greater than 10% |
2. Verification of the Wave number scale:
Carry out calibration using standard polystyrene film and verify the following Wave numbers (in cm-1).
| Std. Wavelength | Observed Wavelength (cm-1) | Acceptance Criteria |
| 3060.0 cm-1 | 3059.0 – 3061.0 cm-1 | |
| 2849.5 cm-1 | 2848.5 – 2850.5 cm-1 | |
| 1942.9 cm-1 | 1941.9 – 1943.9 cm-1 | |
| 1601.2 cm-1 | 1600.2 – 1602.2 cm-1 | |
| 1583.0 cm-1 | 1582.0 – 1584.0 cm-1 | |
| 1154.5 cm-1 | 1153.5 – 1155.5 cm-1 | |
| 1028.3 cm-1 | 1027.3 – 1029.3 cm-1 |
3. Reproducibility of the Wave number:
| Wave number (cm-1) | No.1(cm-1) | No.2(cm-1) | Error(cm-1) | Tolerance(cm-1) |
| 2849.5 | ± 5.0 | |||
| 1601.2 | ± 1.0 | |||
| 1028.3 | ± 1.0 |
The results Complies / Does not complies as per acceptance criteria.
Performed By: ________________ Checked By: ______________
Date: ________________ Date: ______________
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