{"id":817,"date":"2025-06-14T17:02:58","date_gmt":"2025-06-14T17:02:58","guid":{"rendered":"https:\/\/pharmacoguide.com\/?p=817"},"modified":"2025-06-14T17:02:58","modified_gmt":"2025-06-14T17:02:58","slug":"sop-title-operation-cleaning-and-calibration-of-centrifuge-apparatus","status":"publish","type":"post","link":"https:\/\/pharmacoguide.com\/?p=817","title":{"rendered":"SOP Title: Operation, Cleaning and Calibration of Centrifuge Apparatus"},"content":{"rendered":"

SOP Title:<\/u><\/strong> Operation, Cleaning and Calibration of Centrifuge Apparatus<\/u><\/strong><\/p>\n

    \n
  1. Objective:<\/strong><\/li>\n<\/ol>\n

    To lay down the procedure for Operation, Cleaning and Calibration of Centrifuge Apparatus.<\/p>\n

     <\/p>\n

      \n
    1. Scope:<\/strong><\/li>\n<\/ol>\n

      This SOP is applicable for Operation, Cleaning and Calibration of Centrifuge Apparatus located in Quality Control Laboratory.<\/p>\n

      \u00a0<\/strong><\/p>\n

        \n
      1. Responsibility:<\/strong><\/li>\n<\/ol>\n
          \n
        • Quality Control: To prepare and review the SOP. To follow the procedures laid down for Operation, Cleaning and Calibration of Centrifuge Apparatus as per this SOP.<\/li>\n
        • Engineering Department: To carry out preventive maintenance as per schedule and procedure defined.<\/li>\n
        • Quality Assurance Department: To review and approve the SOP and Annexures.<\/li>\n<\/ul>\n

          \u00a0<\/strong><\/p>\n

            \n
          1. Accountability:<\/strong><\/li>\n<\/ol>\n

            Head Quality Control Department, Head Quality Assurance Department.<\/p>\n

             <\/p>\n

              \n
            1. Procedure:<\/strong><\/li>\n<\/ol>\n

              \u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
              \u00a0\u00a0\u00a0 5.1 <\/strong><\/td>\nSafety Precautions\/Instructions:<\/strong><\/td>\n<\/tr>\n
              5.1.1<\/td>\nEnsure all the electrical connections are properly earthed. Ensure the power plug of the centrifuge apparatus is fixed in the socket properly.<\/td>\n <\/td>\n<\/tr>\n
              5.1.2<\/td>\nEnsure that the ON\/OFF switch is working properly by Switching \u00a0\u201cON\u201d<\/p>\n

              the centrifuge apparatus.<\/td>\n

              		 <\/td>\n<\/tr>\n
              \u00a0\u00a0\u00a0 5.2<\/strong><\/td>\nOperation:<\/strong><\/td>\n<\/tr>\n
              5.2.1<\/td>\nSwitch ON the main switch. Red light indicator on the equipment will glow.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.2<\/td>\nOpen the lid of centrifuge apparatus.<\/td>\n <\/td>\n<\/tr>\n
              5.2.3<\/td>\nFour-centrifuge tube stands are provided inside the equipment.<\/td>\n <\/td>\n<\/tr>\n
              5.2.4<\/td>\nCheck that the centrifuge tubes are clean and dry before pouring sample solution in it.<\/td>\n <\/td>\n<\/tr>\n
              5.2.5<\/td>\nPut the centrifuge tube containing sample solution in the centrifuge apparatus.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.6<\/td>\nEnsure that the sample solution should not be more than 80% capacity of the centrifuge tube.<\/td>\n <\/td>\n<\/tr>\n
              5.2.7<\/td>\nPlace the centrifuge tubes in such positions that the opposite sides are balanced.<\/td>\n <\/td>\n<\/tr>\n
              5.2.8<\/td>\nWhen using two centrifuge tubes they should be placed opposite to each other.<\/td>\n <\/td>\n<\/tr>\n
              5.2.9<\/td>\nTurn the main knob to the \u20181\u2019 position in clockwise direction.<\/td>\n <\/td>\n<\/tr>\n
              5.2.10<\/td>\nInstrument will start at low speed (1000 rpm). Then increase the speed to position 2 (2000 rpm), 3 (4000 rpm), 4 (8000 rpm), 5 (10000 rpm) or as required.<\/td>\n <\/td>\n<\/tr>\n
              5.2.11<\/td>\nAfter completion, slowly decrease the speed on the knob and then finally bring to OFF position.<\/td>\n <\/td>\n<\/tr>\n
              5.2.12<\/td>\nOpen the lid of centrifuge machine, remove the centrifuge tube and check for satisfactory results.<\/td>\n <\/td>\n<\/tr>\n
              5.2.13<\/td>\nSwitch OFF the main switch after completion.<\/td>\n <\/td>\n<\/tr>\n
              5.3<\/strong><\/td>\nCleaning Procedure:<\/strong><\/td>\n<\/tr>\n
              5.3.1<\/td>\nClean the centrifuge machine externally and internally with clean lint free cloth.<\/td>\n <\/td>\n<\/tr>\n
              5.4<\/strong><\/td>\nCalibration Procedure:<\/strong><\/td>\n<\/tr>\n
               <\/td>\nFrequency: <\/strong>\u00a0<\/strong>Once in a year by external agency or after carrying out any major maintenance work.<\/td>\n<\/tr>\n
              5.4.1<\/td>\nCalibration is carried out by external agency as per SOP \u201cCalibration of Instruments and Equipments\u201d.<\/td>\n <\/td>\n<\/tr>\n
              5.4.2<\/td>\nRefer Annexure for calibration test to be performed and acceptance criteria.<\/p>\n\n\n\n\n\n\n\n\n\n\n
              Sr. No.<\/strong><\/td>\nTests<\/strong><\/td>\nKnob<\/strong><\/td>\nSet value<\/strong><\/td>\nAcceptance Criteria<\/strong><\/p>\n

              (\u00b1 5%)<\/strong><\/td>\n<\/tr>\n

              1.<\/td>\nRPM Verification<\/td>\n1<\/td>\n1000<\/td>\n1000 \u00b1 50 rpm<\/td>\n<\/tr>\n
              2<\/td>\n2000<\/td>\n2000 \u00b1 100 rpm<\/td>\n<\/tr>\n
              3<\/td>\n4000<\/td>\n4000 \u00b1 200 rpm<\/td>\n<\/tr>\n
              4<\/td>\n8000<\/td>\n8000 \u00b1 400 rpm<\/td>\n<\/tr>\n
              5<\/td>\n10000<\/td>\n10000 \u00b1 500 rpm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n <\/td>\n<\/tr>\n
              5.4.3<\/td>\nGenerate the A. R. No. on the \u201cInstrument\/Equipment Calibration Register\u201d as per SOP and give requisition to QA department for calibration formats of instrument\/equipment on the due date of calibration.<\/td>\n <\/td>\n<\/tr>\n
              5.4.4<\/td>\nFor issuance of calibration report refer SOP \u201cControl of documents, Preparation, Approval, Issuance and Maintenance.<\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

              \u00a0<\/strong><\/p>\n

                \n
              1. Definitions <\/strong>\/ Abbreviations:<\/strong><\/li>\n<\/ol>\n

                \u00a0<\/strong><\/p>\n

                  \n
                • Definitions:<\/strong><\/li>\n<\/ul>\n

                  \u00a0<\/strong><\/p>\n

                    \n
                  • Breakdown: Any activity leading to operation of instrument\/equipment other than the set parameters or unusual sound or vibration observed in the instrument\/equipment.<\/li>\n
                  • Calibration: Tests performed to ensure that the instrument\/equipment is functioning as per set parameters. These tests are performed as per predefined frequency and also after any major repair or replacement of parts.<\/li>\n<\/ul>\n

                    \u00a0<\/strong><\/p>\n

                      \n
                    • Abbreviations:<\/strong><\/li>\n<\/ul>\n

                      \u00a0<\/strong>\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
                      Abbreviation<\/strong><\/td>\nExpansion<\/strong><\/td>\n<\/tr>\n
                      SOP<\/td>\nStandard Operating Procedure<\/td>\n<\/tr>\n
                      QC<\/td>\nQuality Control<\/td>\n<\/tr>\n
                      QA<\/td>\nQuality Assurance<\/td>\n<\/tr>\n
                      No.<\/td>\nNumber<\/td>\n<\/tr>\n
                      A. R. No.<\/td>\nAnalytical Reference Number<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

                       <\/p>\n

                      *Note – Ready to use SOP available in \u201cDOWNLOAD\u201d Section.<\/u><\/em><\/strong><\/p>\n

                       <\/p>\n","protected":false},"excerpt":{"rendered":"

                      SOP Title: Operation, Cleaning and Calibration of Centrifuge Apparatus Objective: To lay down the procedure for Operation, Cleaning and Calibration of Centrifuge Apparatus.   Scope: This SOP is applicable for Operation, Cleaning and Calibration of Centrifuge Apparatus located in Quality Control Laboratory. \u00a0 Responsibility: Quality Control: To prepare and review the SOP. To follow the […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"templates\/template-full-width.php","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-817","post","type-post","status-publish","format-standard","hentry","category-qc-sop"],"_links":{"self":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/817","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=817"}],"version-history":[{"count":1,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/817\/revisions"}],"predecessor-version":[{"id":818,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/817\/revisions\/818"}],"wp:attachment":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=817"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=817"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=817"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}