{"id":759,"date":"2025-06-14T08:39:09","date_gmt":"2025-06-14T08:39:09","guid":{"rendered":"https:\/\/pharmacoguide.com\/?p=759"},"modified":"2025-06-14T08:39:09","modified_gmt":"2025-06-14T08:39:09","slug":"sop-title-operation-and-cleaning-of-laminar-air-flow-bench","status":"publish","type":"post","link":"https:\/\/pharmacoguide.com\/?p=759","title":{"rendered":"SOP Title: Operation and Cleaning of Laminar Air Flow Bench"},"content":{"rendered":"

SOP Title: <\/u><\/strong>Operation and Cleaning of Laminar Air Flow Bench<\/u><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

    \n
  1. Objective:<\/strong><\/li>\n<\/ol>\n

    To lay down the procedure for Operation and Cleaning of the Laminar Air Flow Bench.<\/p>\n

     <\/p>\n

      \n
    1. Scope:<\/strong><\/li>\n<\/ol>\n

      This SOP is applicable for Operation and Cleaning of Laminar Air Flow Bench located in the Microbiology Section.<\/p>\n

        \n
      1. Responsibility:<\/strong><\/li>\n<\/ol>\n
          \n
        • Quality Control: To prepare and review the SOP. To follow the procedures laid down for Operation and Cleaning of Laminar Air Flow Bench as per this SOP.<\/li>\n
        • Engineering Department: To carry out preventive maintenance as per schedule and procedure defined.<\/li>\n
        • Quality Assurance Department: To review and approve the SOP and Annexures.<\/li>\n<\/ul>\n

          \u00a0<\/strong><\/p>\n

            \n
          1. Accountability:<\/strong><\/li>\n<\/ol>\n

            Head Quality Control Department, Head Quality Assurance Department.<\/p>\n

             <\/p>\n

              \n
            1. Procedure:<\/strong><\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
              \u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              \u00a0\u00a0\u00a0 5.1<\/strong><\/td>\nSafety Precautions\/Instructions:<\/strong><\/td>\n<\/tr>\n
              5.1.1<\/td>\nEnsure all the electrical connections are properly earthed. Ensure the power plug of the LAF is fixed in the socket properly.<\/td>\n <\/td>\n<\/tr>\n
              5.1.2<\/td>\nWear personal protective equipment\u2019s such as protective hand gloves, nose masks and over gown during operation of equipment.<\/td>\n <\/td>\n<\/tr>\n
              5.1.3<\/td>\nNever work under LAF when the UV lamp is ON.<\/td>\n <\/td>\n<\/tr>\n
              5.1.4<\/td>\nHEPA filters must never be touched by personnel and no attempt should be made to clean or disinfect filters using chemical or mechanical methods.<\/td>\n <\/td>\n<\/tr>\n
              5.1.5<\/td>\n\u00a0Do not disturb the air flow or switch off the instrument if analyses are not completed.<\/td>\n <\/td>\n<\/tr>\n
              5.1.6<\/td>\nAvoid direct exposure of UV lights on skin and body parts.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.1.7<\/td>\nDispose pre-filters if they are dirty in appearance or when the Magnehelic gauze pressure shows less than half of its initial reading.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              \u00a0\u00a0\u00a0 5.2<\/strong><\/td>\nOperation\u00a0 Procedure:<\/strong><\/td>\n<\/tr>\n
              5.2.1<\/td>\nSwitch \u2018ON\u2019 the equipment<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.2<\/td>\nPut \u2018ON\u2019 the switch of air flow blower.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.3<\/td>\n\u00a0Check the pressure on magnehelic gauze on the top of the equipment should be within the specified limits (between 05-15 mm).<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.4<\/td>\nRecord the reading in Annexure.<\/td>\n <\/td>\n<\/tr>\n
              5.2.5<\/td>\nIf the reading is outside the operating limits, the Microbiologist should inform Quality Control Head immediately.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.6<\/td>\nAfter investigation and rectification, analysis to be carried out.<\/td>\n <\/td>\n<\/tr>\n
              5.2.7<\/td>\n\u00a0Put \u2018ON\u2019 the switch of UV light and close the cover of LAF unit and keep UV lamp ON for 30 minutes. Avoid direct view of burning of UV light.<\/td>\n <\/td>\n<\/tr>\n
              5.2.8<\/td>\nAfter 30 minutes switch \u2018OFF\u2019 the UV Light and put \u2018ON\u2019 the switch of tube light.<\/td>\n <\/td>\n<\/tr>\n
              5.2.9<\/td>\nMaintain the record for UV Lamp burning hour in Annexure.<\/td>\n <\/td>\n<\/tr>\n
              5.2.10<\/td>\nCare to be taken the UV tube burning hours should not exceed 2000 hrs.<\/td>\n <\/td>\n<\/tr>\n
              5.2.11<\/td>\nClean the laminar air flow Bench and side arms with 70 % Isopropyl alcohol. Record the data on Annexure.<\/td>\n <\/td>\n<\/tr>\n
              5.2.12<\/td>\n\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Switch ON LPG cylinder and rotate knob clock wise direction and light burner with lighter. Perform all the microbiological work\/analysis under LAF.<\/td>\n <\/td>\n<\/tr>\n
              5.2.13<\/td>\n\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 After the analysis, switch OFF the burner, by rotating the knob in anticlock direction<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.2.14<\/td>\n\u00a0 \u00a0 \u00a0 \u00a0 \u00a0Switch OFF the LAF Unit.<\/td>\n <\/td>\n<\/tr>\n
              5.2.15<\/td>\nClean up any spillage immediately and sanitize the work surface, panels and surrounding area with 70 % IPA. Switch off tube light.<\/td>\n <\/td>\n<\/tr>\n
              5.2.16<\/td>\nSwitch OFF the main LPG connection<\/td>\n <\/td>\n<\/tr>\n
              5.2.17<\/td>\nPut \u2018ON\u2019 the switch of UV light and keep it ON for 30 minutes.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              \u00a0\u00a0\u00a0 5.3<\/strong><\/td>\nCleaning Procedure:<\/strong><\/td>\n<\/tr>\n
              \u00a0<\/strong><\/td>\nFrequency: <\/strong>Daily twice i.e. before starting and after the completion of analysis.<\/td>\n<\/tr>\n
              5.3.1<\/td>\nDe-dust the external surface of LAF with lint free cloth.<\/td>\n <\/td>\n<\/tr>\n
              5.3.2<\/td>\nBefore switching the LAF Unit \u2018ON\u2019, clean the LAF bench, side glass and grills with lint free nylon cloth damped with 70 % v\/v IPA daily.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.3.3<\/td>\n\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Clean the pre-filters & UV light fortnightly carried out by Maintenance department.<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              \u00a0\u00a0 5.4<\/strong><\/td>\nValidation:<\/strong><\/td>\n<\/tr>\n
               <\/td>\nFrequency: <\/strong>Once in a year or after carrying out any major maintenance work<\/td>\n<\/tr>\n
              5.4.1<\/td>\n\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 For Validation of LAF refer SOP \u2018Procedure for validation of LAF\u2019.<\/td>\n <\/td>\n<\/tr>\n
              \u00a0\u00a0 5.5<\/strong><\/td>\nPreventive Maintenance:<\/strong><\/td>\n<\/tr>\n
              5.5.1<\/td>\nPerform the preventive maintenance as per Annexure<\/td>\n\u00a0<\/strong><\/td>\n<\/tr>\n
              5.5.2<\/td>\nIf preventive maintenance activity is outsourced, the observations are to be transcribed in the Annexure and external party certificate to be attached.<\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

              \u00a0<\/strong>\u00a0<\/strong><\/p>\n

                \n
              1. Definitions <\/strong>\/ Abbreviations:<\/strong><\/li>\n<\/ol>\n
                  \n
                • Definitions:<\/strong><\/li>\n
                • Breakdown: Any activity leading to operation of equipment other than the set parameters and\/ or unusual sound or vibration observed in the equipment.<\/li>\n
                • Calibration: Tests performed to ensure that the equipment is functioning as per set parameters. These tests are performed as per predefined frequency and also after any major repair or replacement of parts.<\/li>\n
                • Preventive Maintenance: Maintenance activity performed to ensure that the equipment will function smoothly and to avoid breakdowns. These activities are performed as per predefined frequency.<\/li>\n<\/ul>\n

                  \u00a0<\/strong><\/p>\n

                    \n
                  • Abbreviations:<\/strong><\/li>\n<\/ul>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
                    Abbreviation<\/strong><\/td>\nExpansion<\/strong><\/td>\n<\/tr>\n
                    LAF<\/td>\nLaminar Air Flow<\/td>\n<\/tr>\n
                    HEPA<\/td>\nHigh Efficiency Particulate air<\/td>\n<\/tr>\n
                    UV<\/td>\nUltra Violet<\/td>\n<\/tr>\n
                    hrs<\/td>\nHours<\/td>\n<\/tr>\n
                    LPG<\/td>\nLiquid Petroleum Gas<\/td>\n<\/tr>\n
                    No.<\/td>\nNumber<\/td>\n<\/tr>\n
                    %<\/td>\nPercent<\/td>\n<\/tr>\n
                    v\/v<\/td>\nVolume by volume<\/td>\n<\/tr>\n
                    IPA<\/td>\nIso Propyl Alcohol<\/td>\n<\/tr>\n
                    PM<\/td>\nPreventive Maintenance<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

                    \u00a0<\/strong><\/p>\n

                    *Note – Ready to use SOP available in \u201cDOWNLOAD\u201d Section.<\/u><\/em><\/strong><\/p>\n

                    \u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

                    SOP Title: Operation and Cleaning of Laminar Air Flow Bench \u00a0 Objective: To lay down the procedure for Operation and Cleaning of the Laminar Air Flow Bench.   Scope: This SOP is applicable for Operation and Cleaning of Laminar Air Flow Bench located in the Microbiology Section. Responsibility: Quality Control: To prepare and review the […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"templates\/template-full-width.php","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-759","post","type-post","status-publish","format-standard","hentry","category-microbiology"],"_links":{"self":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/759","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=759"}],"version-history":[{"count":1,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/759\/revisions"}],"predecessor-version":[{"id":760,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=\/wp\/v2\/posts\/759\/revisions\/760"}],"wp:attachment":[{"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=759"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=759"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmacoguide.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}